Potential Binding Partners for H1 histone in the yeast specie, Saccharomyces cerevisiae

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Live Poster Session: Zoom Link
Thursday, July 30th 1:15-2:30pm EDT

Alaa Narch
Alaa Narch

Alaa Narch is a rising junior (’22). He’s an international student from the green mountains of Lebanon. He graduated from Baakleen High School, the only student to have gone to the US on a full ride in generations. He is an avid gamer who believes video games are a medium for storytelling and education. Wanting to go into the medical field with a holistic understanding of science and health disparities, he is double majoring in science in society and molecular biology and biochemistry.

Anissa Findley
Anissa Findley

Anissa Findley is a Wesleyan student of the Class of 2022. She hails from Kingston, Jamaica where she graduated from the Immaculate Conception High School. Her current majors are Chemistry and Molecular Biology and Biochemistry. In the future, she plans to complete the BA/MA program at Wesleyan and later pursue her PhD.

Abstract: The nucleosome is the basic unit of chromatin which folds to form chromosomes, the final structure in which DNA is packaged in the cell. Nucleosomes consist of DNA wrapped around an octamer of four histone proteins, resulting in a bead on a string type structure. However, our focus is on a fifth histone, H1, which has two domains GI and GII. H1 functions as a linker histone that binds to the linker DNA between each nucleosome. This research centers around unbiased screening of the 6000 genes of budding yeast, Saccharomyces Cerevisiae, for physical interactions with H1 and its GII domain using the yeast two-hybrid assay. The assay currently shows 22 possible interactions with the full-length H1 protein and the GII domain. This research is a preliminary measure for further understanding the functions of the H1 protein and its GII domain. The proteins identified would undergo further screening using different methods to ensure an interaction with the H1 protein or its GII domain.

Summer-Poster-2020-1-Alex-Narsh

Live Poster Session: Zoom Link
Thursday, July 30th 1:15-2:30pm EDT

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